Figure 2. OTX2 activation of the human TYR promoter is mediated through the proximal region at −462 to −152. Luc-reporter constructs containing the human TYR promoter −2525 with enhancer element −9860 to −8741 (=Enh./-2525), deletions −2525, −1995, −462, −152, and promoterless pGL3-Basic were cotransfected into ARPE-19 cells with MITF-A, OTX2, or an empty expression vector (pCR3). Normalized luciferase activities are relative to the TYR promoter construct −152 (=1) cotransfected with the empty expression vector (pCR3). Data are means±standard error of the mean (SEM) from two independent transfections each performed in triplicate.