Appendix 6 of Reinisalo, Mol Vis 2012; 18:38-54.

Appendix 6.

OTX2 activates the tyrosinase promoter through the specific OTX2 binding elements 1 (−361 to −356), 2 (−354 to −349) and 3 (−226 to −221) and through the protein–protein interactions with MITF. To access the data, click or select the words “Appendix 6.” This will initiate the download of a compressed (pdf) archive that contains the file. Luc-reporter constructs containing deletions of the OTX2 binding elements 1, 2 and/or 3 (renamed as m1, m2 and m3) in the human tyrosinase promoter −462 were co-transfected into D407 cells with MITF-A, OTX2, MITF-A/OTX2 factors or with empty expression vector (pCR3). Normalized luciferase activities are relative to tyrosinase promoter construct −152 (=1) co-transfected with empty expression vector pCR3 (not shown). Data are means±SEM from two independent transfections performed in triplicate.