Appendix 5 of Reinisalo, Mol Vis 2012; 18:38-54.
In the RPE, OTX2 activates the tyrosinase promoter in the absence of MITF binding elements. To access the data, click or select the words “Appendix 5.” This will initiate the download of a compressed (pdf) archive that contains the file. Luc-reporter constructs containing deletions of the MITF binding elements at positions −104 to −99 (mM=deletion of the M-box) and −12 to −7 (mE=deletion of the E-box) in the human tyrosinase promoters −152 or −462 were co-transfected into D407 cells with MITF-A, OTX2, MITF-A/OTX2 factors or with empty expression vector (pCR3). Normalized luciferase activities are relative to tyrosinase promoter construct −152 (=1) co-transfected with empty expression vector pCR3 (=vector only). Data are means±SEM from two independent transfections performed in triplicate.