Appendix 4 of Reinisalo, Mol Vis 2012; 18:38-54.

Appendix 4.

OTX2 activates the tyrosinase promoter through the proximal region at −462 to −152. To access the data, click or select the words “Appendix 4.” This will initiate the download of a compressed (pdf) archive that contains the file. Luc-reporter constructs containing the human tyrosinase promoter −2525 with enhancer element −9860 to −8741 (=Enh./-2525), deletions −2525, −1995, −462, −152 and promoterless pGL3-Basic were cotransfected into D407 RPE cells with MITF-A, OTX2 or empty expression vector (pCR3). Normalized luciferase activities are relative to tyrosinase promoter construct −152 (=1) co-transfected with empty expression vector (pCR3). Data are means±SEM from two independent transfections performed in triplicate.