Supplemental methods. Cloning of the human tyrosinase promoter and construction of promoter deletions; and Preparation of nuclear extracts and EMSA reactions. To access the data, click or select the words “Appendix 1.” This will initiate the download of a compressed (pdf) archive that contains the file. Complementary antisense primers for mutagenesis are not shown. Mutated nucleotides are shown with capital letters and the positions of binding sites are underlined.