Figure 7. MicroRNA-34 b/c downregulated c-Met, p-Akt and cell cycle proteins in uveal melanoma cells. SP6.5 cells were transfected with miR-34b/c or a negative control. Cell lysates were prepared and used for western blot analysis with c-Met, phosphorylated Akt (p-Akt), total Akt, phosphorylated ERK1/2 (p-ERK1/2), total ERK1/2, CDK4, CDK6, and phosphorylated-Rb (p-Rb) antibodies. Glyceraldehyde-3-phosphate dehydrogenase was used as a loading control. A: miR-34b/c downregulated the expression of c-Met and p-Akt. B: Cell cycle–related proteins CDK4, CDK6, and p-Rb were downregulated by miR-34b/c.