Figure 6. c-Met was a target of microRNA-34 b/c. A: Two specific binding sites of miR-34b/c in the c-Met 3′ untranslated region (UTR) was marked with black color. Alignment between the predicted miR-34b/c target sites and miR-34b/c, the common 8 bp seed sequence for miR-34b/c:mRNA (mRNA) pairing is shown. B: Design of the pMIR luciferase reporter constructs, containing c-Met 3′ UTR, which was used to verify the putative miR-34b/c binding sites. C: SP6.5 cells were cotransfected with miR-34b/c, pLuc-MET 3′ UTR, and a pRL-SV40 reporter plasmid. The luciferase activity was measured after 24 h. Values are presented as relative luciferase activity after normalization to Renilla luciferase activity. *: Differences in luciferase activity between miR-34b/c and negative control transfected cells were significant, p<0.01.