Figure 5. Transfection of microRNA-34 b/c reduced uveal melanoma cell migration. Transwell migration assay of uveal melanoma cell lines was performed. SP6.5 cells were transfected with miR-34b/c or a negative control (NC) for 24 h and plated on cultured inserts in DMEM containing 20 ng/ml of hepatocyte growth factor (HGF) to assess the number of migrating cells. The number of cells that had migrated through the pores was quantified by counting 10 independent visual fields using a 20× microscope objective. *: Differences in cell migration between miR-34b/c and negative control transfected cells were significant, p<0.01.