Figure 3. Ectopic microRNA-34 b/c
inhibited SP6.5 cell proliferation. A: MTS cell
proliferation assay was performed on days 1 to 5 as indicated
after transfection into SP6.5 cells with either miR-34 b/c mimic
or a negative control (NC). Results represent those obtained in
three experiments. B: SP6.5 cells transfected with
miR-34b/c or NC were seeded at low density. Colony formation was
observed by staining with crystal violet after seven days.
Typical results from three independent experiments are depicted.
C: SP6.5 cells were transfected with miR-34b/c or NC.
After 48 h, cells were collected, stained with propidium iodide,
and analyzed by flow cytometry. The most representative results
from three independent experiments are shown.