Figure 3. Ectopic microRNA-34 b/c inhibited SP6.5 cell proliferation. A: MTS cell proliferation assay was performed on days 1 to 5 as indicated after transfection into SP6.5 cells with either miR-34 b/c mimic or a negative control (NC). Results represent those obtained in three experiments. B: SP6.5 cells transfected with miR-34b/c or NC were seeded at low density. Colony formation was observed by staining with crystal violet after seven days. Typical results from three independent experiments are depicted. C: SP6.5 cells were transfected with miR-34b/c or NC. After 48 h, cells were collected, stained with propidium iodide, and analyzed by flow cytometry. The most representative results from three independent experiments are shown.