Figure 2. MicroRNA-34b/c was upregulated by doxorubicin and epigenetic drugs. A: Real time reverse transcriptase (RT)-PCR analysis was performed to detect the expression of miR-34b/c in uveal melanoma cell line SP6.5, after treatment with DOX for 24 h and 48 h. B: SP6.5 cells were treated with 5-aza-dC at 1 μM or 5 μM alone, TSA (100 ng/ml) alone, or combinations of both. miR-34b/c expression level was determined by Real-time RT–PCR. The value for miR-34b/c in SP6.5 cells without any treatment was set at 1, and the relative amounts of miR-34b/c in cells treated with drugs were shown as fold induction. U6 snRNA was used as an internal control.