Figure 1. MicroRNA-34b/c expression was downregulated in human uveal melanoma cell line and clinical samples. A: Real time reverse transcriptase (RT)-PCR analysis was performed to detect the expression of miR-34b/c in clinical samples. The value for miR-34b/c in normal uveal tissue was set at 1, and the relative amounts of miR-34b/c in tumor tissues were shown as fold induction. Both miR-34b and miR-34c were dramatically decreased in five specimens as compared with normal tissues. N: normal uveal tissue; T: tumor tissue. B: The expression of miR-34b/c was measured by real time RT–PCR in uveal melanoma cell line SP6.5, as well as the primary uveal melanocytes. miR-34b/c was expressed in uveal melanocytes but dramatically decreased in uveal melanoma cells. U6 snRNA was used as an internal control.