Figure 9. Tfe3 knockdown leads to a reduction in vascular endothelial growth factor (VEGF) expression. Human retinal pigment epithelium (ARPE-19) cells were transfected with an siControl or a SMARTpool targeting Tfe3. A: A 20% reduction in VEGF expression and (B) a three- to fourfold increase in Tfe3 downstream targets TRP-1 and tyrosinase were observed with Tfe3 depletion. The experiment was performed in triplicate. A representative experiment is shown with data expressed as the mean±standard deviation. *: p<0.05; **: p<0.01.