Figure 5. Vascular endothelial growth factor (VEGF) promoter regions involved in regulating VEGF expression in RPE. (A) Human retinal pigment epithelium cell line (ARPE-19) or (B) HEK 293 cells were transfected with VEGF-luciferase constructs containing various truncations of the VEGF promoter. There was a twofold induction of promoter activity with the 9 kb construct over the 5.2 kb construct in (A) ARPE-19 cells. In (B) HEK 293 cells, maximum promoter activity was achieved with the 2.1 kb construct. The experiment was performed in triplicate. A representative experiment is shown with data expressed as the mean±standard deviation.