Figure 4. Schematic of vascular
endothelial growth factor (
VEGF)-luciferase reporter
constructs.
VEGF-luciferase constructs indicating the
size of upstream
VEGF promoter sequence, transcription
start site, and 5′ UTR.
VEGF genomic DNA was ligated
upstream of a promoterless luciferase gene in the pGL2-basic
plasmid. Deletions were made by taking advantage of the
restriction sites located within the
VEGF promoter and
the pGL2 multiple cloning site [
16].