Figure 4 of Ford, Mol Vis 2012; 18:519-527.

Figure 4. Schematic of vascular endothelial growth factor (VEGF)-luciferase reporter constructs. VEGF-luciferase constructs indicating the size of upstream VEGF promoter sequence, transcription start site, and 5′ UTR. VEGF genomic DNA was ligated upstream of a promoterless luciferase gene in the pGL2-basic plasmid. Deletions were made by taking advantage of the restriction sites located within the VEGF promoter and the pGL2 multiple cloning site [16].