Table 1 of
Park, Mol Vis 2012; 18:488-494. Table 1 of
Park, Mol Vis 2012; 18:488-494.
Table 1. List of primers used to perform amplification and sequencing of the 14 PAX6 exons.
| PAX6 gene | Primer sequences for PAX6 (5′→3′) | Tm (°C) |
|---|---|---|
| Exon 1 | F-CTCATTTCCCGCTCTGGTTC | 56 |
| R-AAGAGTGTGGGTGAGGAAGT | ||
| Exon 2 | F-TTATCTCTCACTCTCCAGCC | 56 |
| R-AAGCGAGAAGAAAGAAGCGG | ||
| Exon 3 | F-TCAGAGAGCCCATCGACGTAT | 56 |
| R-CTGTTTGTGGGTTTTGAGCC | ||
| Exon 4 | F-TTGGGAGTTCAGGCCTACCT | 56 |
| R-GAAGTCCCAGAAAGACCAGA | ||
| Exon 5 | F-CCTCTTCACTCTGCTCTCTT | 56 |
| R-ATGAAGAGAGGGCGTTGAGA | ||
| Exon 5a | F-TGAAAGTATCATCATATTTGTAG | 50 |
| R-GGGAAGTGGACAGAAAACCA | ||
| Exon 6 | F-GTGGTTTTCTGTCCACTTCC | 56 |
| R-AGGAGAGAGCATTGGGCTTA | ||
| Exon 7 | F-CAGGAGACACTACCATTTGG | 56 |
| R-ATGCACATATGGAGAGCTGC | ||
| Exon 8 | F-GGGAATGTTTTGGTGAGGCT | 56 |
| R-CAAAGGGCCCTGGCTAAATT | ||
| Exon 9 | F-GTAGTTCTGGCACAATATGG | 54 |
| R-GTACTCTGTACAAGCACCTC | ||
| Exon 10 | F-GTAGACACAGTGCTAACCTG | 56 |
| R-CCCGGAGCAAACAGGTTTAA | ||
| Exon 11 | F-TTAAACCTGTTTGCTCCGGG | 56 |
| R-TTATGCAGGCCACCACCAGC | ||
| Exon 12 | F-GCTGTGTGATGTGTTCCTCA | 56 |
| R-TGCAGCCTGCAGAAACAGTG | ||
| Exon 13 | F-CATGTCTGTTTCTCAAAGGGA | 54 |
| R-GAACAATTAACTTTTGCTGGCC |