Figure 3. Halofuginone suppresses TGF-β-induced expression of type I collagen protein and mRNA in human corneal fibroblasts. A: Representative images of immunostainings for type I collagen show cells treated with halofuginone (Hal) and TGF-β (bottom
right) display similar fluorescence signal as untreated control cells (top left), but less signal than cells treated with
TGF-β only (top right). B: qRT–PCR analysis demonstrates treatment with halofuginone significantly reduces both TGF-β-induced expression and basal
expression of COL1A2 mRNA (normalized to ACTB mRNA). * p<0.05 compared to control, ** p<0.05 compared to TGF-β (n=4, bar=S.E.M.).