Figure 4. The effect of Zebularine on the expression of methylation related genes and genes involved in the pathogenesis of PCO. Zebularine treatment was associated with a dose-dependent depletion of DNMT1 in all three doses (10, 50, and 100 μM). Zebularine inhibited the phosphorylation of Akt at serine 473. Phosphorylated p44/42 MAPK level decreased by 87.5% after treatment with high dose (100 μM) Zebularine in 48 h.