Figure 6. Representative confocal slices of Z-series showing Giemsa and PI staining in chicken ossicles. The whole anterior halves of eyes from chicken embryos were stained with both Giemsa and propidium iodide. A: Patterns of the microporosities of chicken scleral ossicles (E16 and E20). B: Features of nuclei in chicken scleral ossicles (E16 and E20). C: Profiles of the ossicle microporosities containing nuclei, indicating that an individual nucleus resides in each bone microporosity (arrows). Scale bar, 50 µm.