Figure 6. K5R expression is
suppressed in the retina of diabetic rats. Diabetes was induced
in adult rats with an injection of STZ and monitored by blood
glucose levels. The rats with blood glucose levels higher than
350 mg/dl were used as diabetic rats. Retinas were isolated from
diabetic rats at 2, 5, and 8 weeks after the STZ injection.
Retinal mRNA levels of K5R were quantified with
real-time RT–PCR and normalized by the 18s RNA level. The
normalized K5R mRNA levels were expressed as % of that
in non-diabetic control (mean±SD, n=3). Values significantly
lower than control are indicated (*p<0.05). Diabetic rats
showed significantly reduced K5R mRNA levels in the
retina, compared to non-diabetic rats.