Figure 4. Western blot results from the family 1 show the absence of Rep-1 protein in the patient (line 2) and a reduced amount of Rep-1 in the female carrier (Line 1). Lane 3 is the normal brother of the patient. A: Mouse anti-REP-1, clone 2F1 demonstrated the absence of Rep-1 protein in the patient and a reduced amount of Rep-1 protein in the female carrier. B: β-actin antibody was used as a loading control to ensure an adequate protein sample in each lane.