Table 1 of Roberts, Mol Vis 2012; 18:280-289.


Table 1. Information pertaining to the primers used to amplify the exons containing the seven most common ABCA4 mutations.


Exon (mutation)

Primer 5′-3′
Annealing
temperature

Mutation detection technique
Exon 5 (p.Arg152*) F: gacccatttccccttcaac 60 °C dHPLC, Cycle sequencing using the reverse primer
  R: aggctgggtgcttccctc    
Exon 6 (c.768G>T) F: ggtgtctttcctaccacag 57.9 °C dHPLC, Cycle sequencing using the forward primer
  R: aggaatcaccttgcaattgg    
Exon 13 (p.Arg602Trp) F: agctatccaagcccgttcc 63 °C SNaPshot PCR
  R: ccattagcgtgtcatggag    
Exon 17 (p.Gly863Ala) F: ctgcggtaaggtaggataggg 60 °C Allele-specific PCR
  R: cacaccgtttacatagagggc    
Exon 30 (p.Cys1490Tyr) F: gtcagcaactttgaggctg 63 °C SNaPshot PCR
  R: tccctctgtggcaggcag    
Intron38/Exon39 (c.5461–10T>C) F: gccccacctgctgaagag 63 °C SNaPshot PCR
  R: tcccagctttggacccag    
Exon 44 (p.Leu2027Phe) F: gaagcttctccagccctagc 63 °C SNaPshot PCR
  R: tgcactctcatgaaacaggc