Figure 3. Overexpression of proNGF reduced NGF and induced expression of TrkA and p75NTR, but not sortilin. A, B: Western blot (WB) analysis of rat retinal lysate showed significant (1.5-fold) increase in expression of TrkA and p75NTR in rats electroporated with pGFP-proNGF123 as compared with those electroporated with pGFP (n=4). C: WB analysis showed that overexpression of pGFP-proNGF123 in rat retinas did not affect sortilin protein expression level
as compared with the control rats (n=4). D: WB analysis showed that overexpression of pGFP-proNGF123 significantly reduced NGF expression (n=5) compared with GFP controls.
The asterisk represents significant difference as compared with control group at p<0.05. ROD, relative optical density.