Table 1 of
Dai, Mol Vis 2012; 18:2947-2953. Table 1 of
Dai, Mol Vis 2012; 18:2947-2953.
Table 1. Sequences of the primers used for reverse transcription polymerase chain reaction.
| Gene name | Nucleotide sequence (5′→3′) sense/anti-sense | Annealing temperature (°C) | Product size (bp) | PCR cycles |
|---|---|---|---|---|
| ARF1 | CGTGTGAACGAGGCCCGTGA | 58 | 190 | 35 |
| TGGTGGCACAGGTGGCCT GA | ||||
| Caspase-3 | ACCGGTGGAGGCTGACTTCCT | 58 | 122 | 35 |
| GCGCGTACAGCTTCAGCATGC | ||||
| VEGF | GAGCGGAGCCGCGAGAAGTG | 58 | 131 | 35 |
| TCCATGAGCCCGGCTTCCGA | ||||
| GAPDH | ACCACAGTCCATGCCATCAC | 56 | 452 | 25 |
| TCCACCACCCTGTTGCTGTA | ||||
| hARF1 | ATCGTGACCACCATTCCCAC | 58 | 408 | 35 |
| GAGCTGATTGGACAGCCAGT |