Figure 1. ADP-ribosylation factor 1 mRNA expression in corneas after alkali injury. A: Semiquantitative reverse transcription polymerase chain reaction (RT–PCR) was performed to evaluate mRNA (mRNA) expression of ARF1. Corneas were harvested at the indicated time points, and five corneas at each time point were pooled to extract total RNAs. Representative results from three independent experiments are shown. B: The relative level of ARF1 gene expression to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was determined. All values represent the mean±standard error of the mean (SEM) of three independent measurements (*p<0.05 versus day 0).