Figure 2. Expression of Occludin and E-cadherin mRNA in human SRA 01/04 and/or mouse αTN4 lens epithelial cells. mRNA expression was analyzed by reverse transcription-polymerase
chain reaction (RT–PCR) using gene specific primers. A: Occludin expression in αTN4 cells demonstrated by amplification of a PCR product of the expected size of 378 base pairs (bp). B: Expression of E-cadherin in SRA 01/04 and αTN4 cells demonstrated by amplification of the expected PCR products, respectively, of 536 and 537 bp.
The specificity of each PCR product was confirmed by sequencing. Molecular sizes of DNA markers in bp are indicated on the
left. M indicates DNA marker and RT- indicates without reverse transcriptase control.