Table 1. List of primers used for
oligonucleotide-directed mutagenesis.
SNP |
Amino
acid
change |
Sequence
|
Tm
(°C ) |
| rs1048661 |
R→L |
F-5’-
gggcatggcccTggcccgcacctcc -3’ |
76 |
| |
|
R-5’-
ggaggtgcgggccAgggccatgccc -3’ |
76 |
| rs3825942 |
G→D |
F-5’-
acggcacggggActccgcctcctcg -3’ |
75 |
| |
|
R-5’-
cgaggaggcggagTccccgtgccgt -3’ |
75 |
Table 1 of
Kim, Mol Vis 2012; 18:265-270.