Figure 1. Construction of the LOXL1 expression constructs for the four different haplotypes. A: A schematic diagram of the recombinant LOXL1 protein expected from the LOXL1 expression constructs. The recombinant LOXL1 protein contains the amino acid sequence from codon 135D to the COOH-terminus with a hexa-histidine tag at the end. The approximate positions of R141L and G153D in the propeptide region are indicated with asterisks. B: DNA sequencing analysis of the mutated LOXL1 expression constructs. The GG haplotype construct (141R-153G) was used as a template for oligonucleotide-directed mutagenesis. The amino acid sequence corresponding to each haplotype is indicated in parentheses. The nucleotide sequences at rs1048661 and rs3825942 are indicated with arrows.