Figure 3. Immunopanning-magnetic separation method for isolating retinal ganglion cells. Retinal cell suspension is incubated with antimacrophage
antibody and distributed over an antiimmunoglobulin G antibody-coated Petri dish. Non-adherent cells are treated with biotinylated
anti-Thy 1 antibody and subsequently interacted with antibiotin magnetic MicroBeads. Finally, the magnetic-labeled retinal
ganglion cells were collected using a column placed in the magnetic field.