Figure 1. Two-step immunopanning method for isolating retinal ganglion cells. Retinal cell suspension is incubated with antimacrophage antibody (Anti-MΦ Ab) and treated in a Petri dish coated with antiimmunoglobulin G antibody (Anti-IgG Ab). Non-adherent cells are then treated in the second Petri dish coated with antithymocyte differentiation antigen 1 (Anti-Thy 1 Ab). Finally, the adherent cells are collected as retinal ganglion cells.