Figure 4. Assessment of corneal opacity. While corneas wounded without prior removal of the epithelium and the limbus (AL/WE, IM/WE) are transparent, corneas wounded after prior removal of the epithelium and limbus and cultured with added TGF-β1 (AL/WT, IM/WT) are opaque. Note that the latter corneas are smaller in diameter due to the total elimination of the limbus by trepanation (8 mm diameter trepan). Scale bars=5 mm.