Figure 8. JNK activity was not associated with SKF83959-induced neuroprotection in H₂O₂-treated cells. A: The JNK inhibitor was unable to attenuate SKF83959-mediated neuroprotection. RGC-5 cells were pretreated with 100 μM SP600125 30 min before addition of 30 μM SKF83959 in the presence of 500 μM H₂O₂ for 5 h. Cell viability was determined with MTT assay. Data were obtained from at least three independent experiments and expressed as the mean±SEM B: Cotreatment with ERK and 38 inhibitors completely abolished SKF83959-mediated neuroprotection. RGC-5 cells were pretreated with 20 μM U0126 and 200 μM SB203580 30 min before 30 μM SKF83959 was added in the presence of 500 μM H₂O₂ for 5 h. Cell viability was determined with MTT assay. Data were obtained from at least three independent experiments and expressed as the mean±SEM ***p<0.001, compared with SKF83959-pretreated cells.