Figure 7. p-JNK levels were not affected by H₂O₂ treatment. RGC-5 cells were treated with 500 μM H₂O₂ for the designated times. Cells were then harvested and lysed for immunoblot assay. A: Treatment with 500 μM H₂O₂ had no significant effect on p-JNK or total JNK levels detected with western blot from 1 h to 5 h relative to the controls. Con: control. Each experiment was repeated at least three times, and a representative blot is shown. B: p-JNK levels were quantitated and statistically analyzed compared to controls. Data were obtained from at least three independent experiments and expressed as the mean±SEM C: Total JNK levels were quantitated and statistically analyzed compared to controls. Data were obtained from at least three independent experiments and expressed as the mean±SEM.