Figure 6. SKF83959 treatment preserved levels of active, p-p38 in H₂O₂-treated RGC-5 cells. RGC-5 cells were treated with various agents for the designated times. Cells were then harvested and lysed for immunoblot assay. A: Pretreatment with 30 μM SKF83959 prevented the H₂O₂-induced reduction of p-p38 levels. The specific inhibitor of p38, 200 μM SB203580, abolished the SKF83959-induced preservation of p-p38 levels. SKF: SKF83959. Each experiment was repeated at least three times, and a representative blot is shown. B: Levels of p-p38 were quantitated and statistically analyzed. Data were obtained from at least three independent experiments and expressed as the mean±SEM ***p<0.001, compared with controls. C: SB203580 attenuated SKF83959-mediated neuroprotection. RGC-5 cells were pretreated with 200 μM SB203580 30 min before 30 μM SKF83959 was added in the presence of 500 μM H₂O₂ for 5 h. Cell viability was determined with MTT assay. Data were obtained from at least three independent experiments and expressed as the mean±SEM ***p<0.001, compared with SKF83959-pretreated cells.