Figure 5. Pluripotency and differentiation potency of human Tenon’s capsule fibroblast derived induced pluripotent stem cells. A: Comparable alkaline phosphatase activity levels were observed in human Tenon’s capsule fibroblast derived induced pluripotent stem (TiPS) cells and human embryonic stem cell (hESC). The graph shows the mean±SD (n=3). B: Quantitative real-time RT–PCR analysis of endogenous Oct4, Sox2, c-Myc, Klf4, and Nanog expression in TiPS clones, hESC clones, and HTFs. Nanog, Oct3/4, and Sox2 were expressed at a higher level in TiPS clones and hESC clones, whereas Klf4 and c-Myc were expressed at a lower level, indicating silencing. Transcript levels were normalized to TBP levels. The graph shows the mean±SD (n=3). C: Teratoma derived from TiPS and hESC. Hematoxylin and eosin staining of the TiPS-derived teratoma, showing derivatives of the three germ layers, including neural rosettes (ectoderm), muscle (mesoderm), and gut-like epithelium (endoderm). hESCs were used as a control. Scale bar=50 μm.