Figure 4. Expression of human embryonic stem cell markers in human Tenon’s capsule fibroblast derived induced pluripotent stem cells. A: Confocal immunofluorescent images of representative human Tenon’s capsule fibroblast derived induced pluripotent stem cells (TiPS) clones stained with the human embryonic stem (hES) markers SSEA-4 (red), TRA-1–60 (green), Nanog (red), and Oct-4 (red). Nuclei were stained with DAPI (blue). hESCs were used as a control. Scale bars=50 μm. B: Flow cytometry analysis of the equivalent SSEA-4 expression levels in TiPS and hESCs. 99.5% and 99.7% SSEA-4 + cells (in the Q4 area), respectively, whereas 0.3% in HTFs (p<0.001).