Figure 7. The effects of 15-LOX-1 overexpression on the retinal levels of VEGF-A in oxygen-induced ischemic retinopathy mice. The experiments were divided into the oxygen-induced retinopathy treated with adenoviral-15-lipoxygenase-1 group (the 15-LOX-1 group), the untreated oxygen-induced retinopathy group (the OIR group), the oxygen-induced retinopathy treated with adenoviral-green fluorescence protein group (the GFP group) and the normal control group (the normal group). All mice were euthanized, and the eyes were enucleated at postnatal day 17. A: Retinal levels of VEGF-A and β-actin were measured with western blot analysis (four mice per experimental group). The expression levels of VEGF-A protein were quantified with densitometry and normalized to β-actin. B: The expression of VEGF-A (means±SD, n=3) was compared for the 15-LOX-1 group, the OIR group, the GFP group, and the normal group using one-way ANOVA (15-LOX-1 group versus OIR group *p<0.05, n=3; 15-LOX-1 group versus GFP group **p<0.05, n=3). C: Real-time PCR analysis of VEGF-A expression showed that the mRNA expression levels of VEGF-A in 15-LOX-1 group were lower than those in the OIR group and the GFP group (three mice per experimental group, one-way ANOVA). The relative amount of mRNA was normalized to β-actin (means±SD, n=3). 15-LOX-1 group versus OIR group *p<0.05, n=3; 15-LOX-1 group versus GFP group **p<0.05, n=3.