Figure 3. Western blot analysis confirmed overexpression of 15-LOX-1. The experiments were divided into the oxygen-induced retinopathy treated with adenoviral-15-lipoxygenase-1 group (the 15-LOX-1 group), the untreated oxygen-induced retinopathy group (the OIR group), the oxygen-induced retinopathy treated with adenoviral-green fluorescence protein group (the GFP group) and the normal control group (the normal group), with four mice per experimental group. Western blot analysis showed that the 15-LOX-1 protein levels were higher in the 15-LOX-1 group mice retinas than those in OIR group, GFP group, and normal group mice retinas. The bands were quantified with densitometry and normalized by β-actin; data were analyzed as means±SD (A, B). 15-LOX-1 group versus OIR group *p<0.05, n=3; 15-LOX-1 group versus GFP group **p<0.05, n=3; 15-LOX-1 group versus Normal group ***p<0.05, n=3.