Figure 2. This figure shows the transgene expression of recombinant adenoviral vectors in the retinas of 12-day-old oxygen-induced retinopathy mice, five days after intravitreous injections of approximately 1.0×10⁹ plaque formation unit of Ad-15-LOX-1 or Ad-GFP (four mice per experimental group). Immunofluorescence staining indicated that the 15-LOX-1 protein is more strongly expressed in retinas from the Ad-15-LOX-1 group (A) mainly in the outer plexiform layer (OPL), inner nuclear layer (INL), and ganglion cell layer (GCL) than in retinas from the Ad-GFP group (B). Cell nuclei were stained with 4', 6-diamidino −2-phenylindole (DAPI). Images A and B were taken at 400× magnification for optimal comparison, scale bars=35 um.