Figure 2. The effect of geldanamycin on the interaction between Hsp90 and the CB2 receptor in trabecular meshwork cells. Intact trabecular meshwork (TM) cells were treated with 10 μM geldanamycin (GA) for 2 h. Membranes were prepared and subjected to coimmunoprecipitation with the anti-CB2 antibody. Then, the samples were immunoblotted with the anti-Hsp90 and anti-CB2 antibodies. The experiment was repeated three times, with similar results as shown in the bar graph. Treatment of TM cells with geldanamycin significantly inhibited the Hsp90 that was coimmunoprecipated by the anti-CB2 antibody.