Figure 1. Identification of Hsp90 as a CB2 receptor interacting protein in trabecular meshwork cells. A: Membranes prepared from trabecular meshwork (TM) cells were subject to immunoprecipitation with the anti-CB2 antibody, and
were then immunoblotted with the anti-Hsp90 antibody. B: Membranes prepared from TM cells were subject to immunoprecipitation with the anti-Hsp90, and were then immunoblotted with
the anti-CB2 antibody. Control experiments were performed using non-relevant IgGs. The experiment was repeated three times,
with similar results. Hsp90 was coimmunoprecipitated by the anti-CB2 antibody, whereas the CB2 receptor was coimmunoprecipitated
by the anti-Hsp90 antibody from solubilized TM cell membrane preparations.