Figure 3. The effects of the blends on expression of collagen type IV in cultivated BCECs. A: Western blot analysis of anti- collagen type IV and anti-β-actin in BCECs cultured on chitosan, PCL 25, PCL 50, and PCL 75 after 7 days of incubation. Increased expression of type IV collagen, a corneal endothelial cell marker, with a molecular weight of 160 KDa was clearly observed in BCECs cultured on blends. B: The relative intensities of collagen type IV level were determined by band densitometry analysis. The ratio of the band intensities was expressed as a percentage of chitosan. Results were expressed as mean±SD from three independent experiments (*p<0.05 or **p<0.01).