Figure 6. Survival of OPN4-expressing intrinsically photosensitive retinal ganglion cells does not depend on phosphatidylinositol 3-kinase/AKT and Janus kinase/signal transducer and activator of transcription signaling. A: Shown is the relative expression of Brn3a and Opn4 in retinas of 129S6 wild type mice at 24 h after injection of N-methyl-D-aspartic acid (NMDA, black bars), NMDA plus wortmannin (WM, grey bars), or WM (white bars). Shown are means±SD of n=4-8. **: p<0.01. One-way ANOVA with Bonferroni post hoc test was used to test statistical significance. B: Shown are levels of proteins and phosphoproteins in total retinal extracts from 129S6 wild type mice at 6 h after injection of NMDA or NMDA plus WM as indicated, n=3. C: Shown is the relative expression of Brn3a and Opn4 in retinas of 129S6 wild type mice at 48 h after injection of NMDA in 50% DMSO (black bars), NMDA plus AG-490 in 50% DMSO (grey bars), or non-injected controls (white bars). Shown are means±SD of n=4. ***: p<0.001. One-way ANOVA with Bonferroni post hoc test was used to test statistical significance. D: Shown are levels of STAT3 and pSTAT3 in total retinal extracts from 129S6 wild type mice at 48 h after injection of NMDA or NMDA plus AG-490 as indicated. C: RNA and D: proteins were simultaneously isolated from the same retinas for analysis, n=4.