Figure 9. Cell proliferation inhibition by aptamer-Dox conjugates in vitro. The Y79 and Müller glial cells were seeded 24 h before treatment with free Dox, and aptamers (EpDT3 and Scr-EpDT3) and aptamer-Dox conjugates (EpDT3-Dox and Scr-EpDT3-Dox; n=3) were added to cells in serum-free condition for 2 h followed by media change with 10% FBS. Cells were further incubated for 24 h and 48 h, and the MTT assay was performed. A: The graph represents the percent viable Y79 cells after 24 h and 48 h of treatment. Cytotoxicity was observed with cells treated with free Dox and EpDT3-Dox. B: Graph showing percentage viability of Müller glial cells treated with aptamer and aptamer-Dox. Only free Dox treated cells exhibit significant cytotoxicity.