Figure 5. Diffusion of doxorubicin from the aptamer-Dox conjugate. The time-dependent release of doxorubicin from aptamer-Dox conjugates was monitored by dialyzing free Dox and aptamer-Dox conjugates against conjugation buffer. Dox from the aptamer-Dox conjugate (closed square and triangle) and the free Dox (closed diamond) released were monitored from 2 to 6 h of the initiation of dialysis and represented as % cumulative release. Aptamer-Dox conjugates release Dox comparatively slower than free Dox.