Figure 5. Bromodeoxyuridin (BrdU) pulse-chase-labeled retinal progenitor cells (RPCs) in transplanted RPCs. Retinal sections of wild-type (A), noninjected (B), and injected eyes (C, D) were immunostained with anti-BrdU antibody at three or six weeks after transplantation (brown). Dashed boxes indicate regions depicted at higher magnification in panels E-H: Arrowheads represent BrdU-positive cells that have incorporated into the ganglion cell layer (GCL), and arrows represent BrdU-positive cells that have not incorporated into the GCL (brown). Sections were counterstained with hematoxylin. Scale bars: A-D: 100 μm; E-H: 50 μm.