Figure 2. Time-dependent changes in the expression of amyloid β_1–42 in retina and optic nerve head after laser photocoagulation treatment in cynomolgus monkeys. Representative photographs of retinal and optic nerve head (ONH) sections immunostained with anti-amyloid β (Aβ_1–42, green) and counterstained with Hoechst 33,342 (blue) were obtained from the monkeys that had the pressure in their left eye elevated at 4 to 24 weeks. Aβ_1–42 immunoreactivity was found to be increased in the nerve fiber layer (NFL), the ganglion cell layer (GCL), and ONH of the glaucomatous left eye from 11 weeks after IOP elevation, while there was no Aβ_1–42 immunoreactivity in the non-treated eye. Abbreviations are as follows: IOP represents intraocular pressure; IPL represents inner plexiform layer; INL represents inner nuclear layer; OPL represents outer plexiform layer. Horizontal scale bars indicate 50 µm.