Table 2 of
Versura, Mol Vis 2012; 18:2526-2537. Table 2 of
Versura, Mol Vis 2012; 18:2526-2537.
Table 2. Tear proteins recognized by 2100 Agilent Bioanalyzer and validated in the study, listed along with their name and code from S-Prot human database, theoretical molecular weight in kDa and function.
| Protein name | Protein name database | Theoretical size (kDa) | 1D SDS–PAGE size (kDa) | Bioanalyzer size range (kDa) Lab-chip Kit | Tear/ plasma derived | Function | ||
|---|---|---|---|---|---|---|---|---|
| 80 | 230 | HS 250 | ||||||
| Lysozyme | LYSC_HUMAN (P61626) | 14 | 13.8–14.5 | 12.8–13.7 | 14.3–15.0 | 14.8–15.1 | Tear | Antibacterial enzyme; destroys the cell walls of certain bacteria. Innate immunity |
| Lipocalin-1 | LCN1_HUMAN (P31025) | 17 | 16.5–17.6 | 16.7–18.4 | 18.1–19.9 | 17.7–18.8 | Tear | Ability to bind small, hydrophobic molecules such as retinol to transport and protect |
| Zinc-a2-glycoprotein (ZAG) | ZA2G_HUMAN (P25311) | 34.2 | 33.0–35.0 | 21.5–24.6 | 30.5–36.0 | 29.7–35.6 | Tear, plasma | It stimulates lipid breakdown in adipocytes, it may have a role in the expression of the immune response, specific role of ZAG in tears not known |
| Albumin | ALBU_HUMAN (P02768) | 68 | 65.0–68.0 | 56.7–57.6 | 59.1–65.4 | 57.1–58.5 | Plasma | Transportation of free fatty acids, stabilizing the osmotic pressure |
| Lactotransferrin | TRFL_HUMAN (P02788) | 82 | 78.0–83.0 | 83.0–84.2 | 93.7–99.3 105.2–110* | 96.5–99.1 | Tear | Tear Inhibitor of bacterial growth possible anti-inflammatory properties. Innate immunity |
| Serotransferrin | TRFE_HUMAN (P02787) | 77 | 70.0–72.0 | 64.8–65.1 | 73.5–78.4 | 86.0–89.2 | Plasma | Iron binding transport proteins, expressed by the liver and secreted in plasma |
| Lipophillin A | SG1D1_HUMAN (O95968) | 9.8 | 8.9–9.2 | 5.2–5.3 | 9.3–11.0 | 12.8–13.0 | Tear | May bind androgens and other steroids, possibly under transcriptional regulation of steroid hormones. |