Table 1 of
Buentello-Volante, Mol Vis 2012;
18:2518-2525. Table 1 of
Buentello-Volante, Mol Vis 2012;
18:2518-2525.
Table 1. Oligonucleotide sequences, amplicon sizes, and annealing temperatures for PCR amplification of AMD-related variants.
| Amplicon | Forward primer (5′-3′) | Reverse primer (5′-3′) | Size PCR product (bp) | Temperature (°C) |
|---|---|---|---|---|
| CFH Y402H | AGTTCGTCTTCAGTTATAC | TGGTCTGCGCTTTTGGAAGAGC | 263 | 59.5 |
| CFB L9H | ACACCATCCTGCCCCAGGCCCA | TTGATCTCTACCCCCTCCAG | 267 | 67.2 |
| CFB R32Q | AGCCCCCAACTCTGCCTGATGCCC | GTACTCCAGTGCCTGGCCCTC | 252 | 65.2 |
| CFB K565E | GCGGGACCTGGAGATAGAAGT | TAGTCTGGCCATATTTCAGC | 140 | 59.1 |
| C2 E318D | GTGAGCGTTGCCATTATCACCT | TGAGAGGGTCCATCTTCTC | 171 | 57.6 |
| C2 A69S | CACTCTGCGAGAGTCTGTGCTGGACC | TTTCTTACCAGTGTCAGGTGG | 201 | 65.4 |
| C3 R102G | GATCTCTTTGCCTCTCCTAA | TCTCCTCTTCTGAACTCCTC | 720 | 56.6 |