Figure 3. Secretion of vascular endothelial growth factor (VEGF)-A and –C. VEGF-A and VEGF-C secretion in ARPE-19 (A, C and HECV B, D cells, respectively, transfected with a scrambled siRNA sequence (siControl) or siRNA of RAGE (siRAGE) following 24 h of culture in standard conditions (CTR) or in the presence of AGEs (GS).The conditioned media were collected, and ELISA was performed. Concentrations of VEGFs were calculated from standards curves and normalized to total protein. The results are representative of at least three experiments (mean±SEM). **p<0.01 and ***p<0.001 versus CTR siControl;^§§ p<0.01 and ^§§§ p<0.001 versus GS siControl;^♦p<0.05 versus CTR siRAGE.