Figure 3. Secretion of vascular
endothelial growth factor (VEGF)-A and –C. VEGF-A and VEGF-C
secretion in ARPE-19 (A, C and HECV B, D
cells, respectively, transfected with a scrambled siRNA sequence
(siControl) or siRNA of RAGE (siRAGE) following 24 h of culture
in standard conditions (CTR) or in the presence of AGEs (GS).The
conditioned media were collected, and ELISA was performed.
Concentrations of VEGFs were calculated from standards curves
and normalized to total protein. The results are representative
of at least three experiments (mean±SEM). **p<0.01 and
***p<0.001 versus CTR siControl;§§
p<0.01 and §§§ p<0.001 versus GS siControl;♦p<0.05
versus CTR siRAGE.