Figure 2. Expression of receptor for AGEs (RAGE). Expression of RAGE in ARPE-19 cells transfected with control siRNA or siRNA of RAGE (siRAGE) following 24 h of culture in standard conditions (CTR) or in the presence of AGEs (GS). Cells were lysed, and lysates were processed for immunoblot. Cells were lysed, and lysates were processed for immunoblot. Bands were visualized with an ECL detection system, and intensity was quantified with densitometric analysis using NIH Image software and expressed as fold induction relative to β-actin. Data shown are representative of at least three experiments (mean±SEM). *p<0.05 versus CTR siControl; ^§p<0.05 versus GS siControl. Comparable results were obtained in HECV (data not shown).